한우의 근출혈 발생 상태에 따른 혈액내 포도당, Creatinine, Lactate, Cortisol 수준과 근육섬유의 조직학적 분석
Blood Glucose, Creatinine, Lactate, and Cortisol Concentration and Histological Analysis of Muscle Fiber in Blood Splash Hanwoo Muscle
This study investigated the stress-related metabolites and hormones in blood and compared the muscle structure to identify the reason for blood splash in Hanwoo beef. Five slaughter houses were selected based on the region (Seoul, Gimhae, Jungbu, Naju, and Goryung) and a previous blood splash record. In total, three-hundred eighty blood samples (n=380) and forty-two muscle tissues (n=42) of control and blood splash Hanwoo beef were collected during the slaughter process and beef grading. Blood metabolites were analyzed including glucose, lactate, creatinine, urea-N, and hormones such as cortisol and thyroxin. Muscle fiber, fiber bundle, and capillary wall thickness were measured under microscope. The concentrations of blood glucose, lactate, and urea-N were not significantly different between the control and the blood splash samples. Cortisol and thyroxin levels were not significantly different in both samples. In contrast, the creatinine level was significantly increased (p<0.05) in the blood splash samples. There were also no significant differences observed in muscle fiber, bundles, and capillary wall thickness between the control and the blood splash tissues. In conclusion, blood metabolites, hormones, and muscle fiber showed no differences between the control and the blood splash animal. However, increased creatinine levels may be used as an indicator for identifying blood splash prior to slaughter in Hanwoo.
목차
ABSTRACT Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 혈액 및 근육 시료 채취 2. 혈액분석 3. 조직 slide 제작 4. 근육 조직 분석 5. 통계분석 Ⅲ. 결과 및 고찰 1. 혈액내 대사물 및 호르몬 2. 근출혈 조직의 현미경적 분석 Ⅳ. 요약 사사 Ⅴ. REFERENCES
저자
선상수 [ Sang Soo Sun | 전남대학교 동물자원학부 ]
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