Earticle

초록

영어

Sparassis latifolia (formerly S. crispa) is used in food and nutraceuticals or dietary supplements, as rich in flavor compounds and β-glucan. Some previous studies have reported the effects of mushroom on brain function, including its neuroprotective effect. Thus, for this mushroom to be used as an effective nutraceutical for brain function, it would be desirable for it to contain other compounds such as γ-aminobutyric acid (GABA) in addition to β-glucan. In this study, the enhancement of growth and GABA production in the mycelium of medicinal and edible mushroom S. latifolia was investigated. Amino acids were added externally as the main source of nutrition, and the effects of amino acids were investigated using liquid medium, specifically amino acid-free potato dextrose broth (PDB). The amino acids added were L-glutamic acid (named PDBG medium) and L-ornithine (named PDBO medium). The growth of mycelia was determined to be 0.9 ± 0.00 g/L, 2.2 ± 0.16 g/L, and 1.93 ± 0.34 g/L PDBG respectively. The GABA content was 21.3 ± 0.9 mg/100 g in PDB medium, and it in PDBG 1.4% medium, at 115.4 ± 30.2 mg/100 g. However, the PDBO medium was not effective in increasing the GABA content of mycelia. Amino acids had little effect on the β-glucan content of mycelia. The β-glucan content was 39.7 ± 1.4 mg/100 mg, 34.4 ± 0.2 mg/100 mg, and 35.2 ± 9.2 mg/100 mg in PDB, PDBG 1.8% and PDBO 1.4% media, respectively. Addition of glutamic acid and ornithine positively affected the growth of S. latifolia mycelia, and glutamic acid positively affected GABA production; no degradation of GABA was observed with addition of glutamic acid.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주 및 생장 확인
  아미노산 첨가 배지 제조
  아미노산 첨가 배지에서의 균사체 배양
  GABA pre-column derivatization
  GABA 함량 측정
  β-glucan 함량 측정
  통계처리
 결과 및 고찰
  꽃송이버섯 균사체 생장 확인
  꽃송이버섯 생장에 미치는 영향
  GABA 생산에 미치는 영향
  β-Glucan 함량에 미치는 영향
 적요
 감사의 글
 References

저자

• 조한교 [ Han-Gyo Jo | 조선대학교 대학원 화학공학과 ]
• 신현재 [ Hyun-Jae Shin | 조선대학교 대학원 화학공학과 ] Corresponding author

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