Earticle

초록

영어

An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof 94.3 mg L-1 was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at 35oC. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.

목차

ABSTRACT
 서론
 재료 및 방법
  균주의 분리
  Auxin 생성균주의 선발
  TLC에 의한 IAA의 확인
  HPLC를 통한 IAA의 정량분석
  Bio assay를 통한 식물생장 실험
  분리 균주의 동정
 결과 및 고찰
  균주의 분리
  Auxin 물질의 확인 및 생산능 비교
  Bio assay를 통한 식물생장 실험
  분리균의 동정
  배양조건별 Auxin 생산능 비교
 결론
 Reference

저자

• Ji-Yeong Yoo [ Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University ]
• Heon-Hak Lee [ Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University ]
• Chang-Hoon Han [ Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University ]
• Min-Ho Yoon [ Department of Bio-Environmental Chemistry, College of Agriculture and Life sciences, Chungnam National University ] Corresponding author

참고문헌