Earticle

초록

영어

Hot water extracts from 16 domestic edible mushrooms including Pleurotus ferulae (Lanzi) X.L. Mao, Lentinula edodes (Berk.) Pegler, and Hypsizygus marmoreus (Peck) H.E. Bigelow, which are commercially available, were used for determining the cosmetic potential of these mushrooms. In this study, we carried out in vitro functional experiments to determine the inhibitory effects of these extracts on L-DOPA oxidation of tyrosinase and melanin synthesis. Based on the results of these experiments, H. marmoreus (Peck) H.E. Bigelow No. 10 and No. 15 were selected for further analysis. We analyzed the melanin synthesis inhibitory activity, TRP1 and MITF expression via real-time PCR, and Fontana Masson staining in artificial skin Neoderm®-ME. Taken together, we observed that the hot water extract from H. marmoreus (Peck) H.E. Bigelow (No. 15) had better whitening effect than the extracts of other mushrooms. Thus, it can be a potential source of skin-whitening agent.

목차

ABSTRACT
 서론
 재료 및 방법
  버섯 열수 추출물 제조
  세포주 배양
  Cell viability 측정
  L-DOPA oxidation inhibitory activity
  Melanin synthesis inhibitory activity
  In vitro test on Artificial skin
  Melanin synthesis inhibitory activity
  Real-time PCR
  Paraffin block 제작
  Fontana-Masson staining
  Statistical analysis
 결과 및 고찰
  Cell viability
  L-DOPA oxidation inhibitory activity
  Melanin synthesis inhibitory activity
  In vitro test on Artificial skin
 적요
 참고문헌

저자

• 김현재 [ Hyunjae Kim | 성균관대학교 식품생명공학과 ]
• 장병현 [ Byunghyun Jang | 성균관대학교 식품생명공학과 ]
• 박건희 [ Kunhee Park | 성균관대학교 식품생명공학과 ]
• 장갑열 [ Gabyeol Jang | 립원예특작과학원 ]
• 박기문 [ Kimoon Park | 성균관대학교 식품생명공학과 ] Corresponding author

참고문헌