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초록

영어

A recent study reported that Pleurotus ostreatus has the potential to be used as a β-glucan- based cream for supportive complementary therapy of atopic dermatitis. KH054 is a new herbal prescription consisting of P. ostreatus and Panax ginseng. The effects of atopic dermatitis-induced materials on the expression of cytokine genes in human monocytes (THP-1, EoL- 1) have been examined. Some reports demonstrated that P. ginseng augments the activity of natural killer cells, which plays an important role in innate immunity against infection and tumor development. Monocyte chemotactic protein 1 (MCP-1), interleukin (IL)-6, and IL-8 have important roles in mediating the infiltration of various cells into the skin of atopic dermatitis and psoriasis. The present study investigated whether KH054 on induced IL-6, IL-8, and MCP-1 secretion by house dust mite (Dermatophagoides pteronissinus) in THP-1 (human acute monocytic leukemia) and EoL-1 (Human eosinophilic leukemia) cell. D. pteronissinus functions in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. The inhibitory effect of KH054 on the induction of IL-6, IL-8, and MCP-1 secretion by D. pteronissinus extract in THP-1 and EoL-1 cells was examined. KH054 potently suppressed the elevated production of IL-6 and IL-8 induced by D. pteronissinus treatment in THP-1 and EoL-1 cells. Based on the present results, KH054 may be useful for developing functional foods to treat atopic dermatitis.

목차

ABSTRACT
 Introduction
 Materials and methods
  D. pteronyssinus preparation
  Preparation of KH054
  Cell culture
  Viability assay
  Enzyme-linked immunosorbent assay (ELISA)
  Quantitative real –time PCR
  Statistical analysis
 Results and Discussion
  KH054 inhibits the secretion of MCP1, IL6 and IL8 in THP1 and EoL1 cells.
  KH054 inhibits the mRNA expression of chemokines and proinflammatory cytokines in LPS-stimulated THP-1 cells
 Summary
 Rererence

저자

• Kyeong Hun Park [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Eun Suk Lee [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Yong Ik Jin [ Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea ]
• Kyung Sun Myung [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Hong Woo Park [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Chun Geon Park [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Won Sik Kong [ Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ]
• Young Ock Kim [ Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Korea ] Corresponding author

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