To obtain information on the sanitary of indoor environment in greenhouses used for shiitake cultivation, bacteria associated with larvae and imagoes of Sciaridae flies, pest to shiitake mushroom, were isolated and identified. A total of 1,048 bacterial colonies were isolated from the flies’ larvae and 984 bacterial colonies were isolated from the flies’ imagoes. Based on molecular analysis of the 16S rDNA sequence, Achromobacter xylosoxidans and Tetrathiobacter kashmirensis of β-proteobacteria, Enterobacter asburiae and Raoultella ornithinolytica of γ -proteobacteria, Curtobacterium sp. and Microbacterium thalassium of Actinobacteria, and Penibacillus taichungensis of Firmicutes were identified from the colonies of the flies’ larvae. While, Bacillus megaterium, B. thuringiensis, Lysinbacillus sphaericus and L. fusifomis of Firmicutes, Microbacterium thalassium and Citricoccus parietis of Actinobacteria, and Enterobacter asburiae of γ-proteobacteria were identified from the flies’ imagoes. Some of the isolated bacterial species were known be human pathogens. Overall, the results of this study suggested that mushroom fly carrying human pathogenic bacteria is one of sources impact on the sanitary of indoor environment of greenhouses used for shiitake cultivation.
저자
Hong Seok Ahn [ Department of Microbiology ]
Hyuk Woo Kwon [ Forest Mushroom Research Center, National Foresty Cooperatives Federation, Yeoju, Gyeonggi, 12653, Korea ]
Yeo Hong Yun [ Department of Microbiology ]
Jun Young Kim [ Department of Microbiology ]
Han Kyu Ko [ Forest Mushroom Research Center, National Foresty Cooperatives Federation, Yeoju, Gyeonggi, 12653, Korea ]
Seong Hwan Kim [ Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan, Chungnam, 31116, Korea ]
