Earticle

초록

영어

This study was conducted to investigate the effects of antiprotozoal plant extracts on in vitro ruminal fermentation and methane emission. Ruminal contents were collected from two ruminally-fistulated steers (Average BW: 450±30 kg) consuming 60% Timothy hay and 40% concentrate with free access to water and mineral block. Plant extracts, Lonicera japonica, Torreya nucifera, Zanthoxylum schinifolium, Poncirus trifoliate and Citrus junos, which have been known to have antiprotozoal and antiparasitic effects were added (5% of substrate) to the in vitro ruminal fermentation bottles containing the mixture of rumen fluid and McDougall’s buffer. The McDougall buffer (1000 ml) and ruminal inoculums (500 ml) were combined and 20 ml added to the 50 ml fermentation vessels containing 300 mg of substrate (DM basis). The fermentation vessels were placed in an incubator at 39°C for 3, 6, 9, 12, 24, 48 and 72 h with shaking. Plant extracts addition was decreased (p<0.05) acetate:propionate compared with control except with T. nucifera addition. Total gas production was increased (p<0.05), whereas methane emission was decreased (p<0.05) by all plant extracts compared with control at 12 h incubation. Methane emission at 24 h incubation was decreased for Lonicera japonica, Torreya nucifera, and Zanthoxylum schinifolium compared with control as well. In addition, methanogenic archaea was decreased (p<0.05) by all plant extracts at the 12 h incubation. These results indicate that the plant extracts may decrease methane emission by inhibiting methanogenesis without the decrease of total gas production in 12 h in vitro ruminal fermentation. Therefore, plant extracts which have the antiprotozoal effect may be promising feed additives for ruminants as a methane inhibitor.

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 공시동물 사양관리 및 시험위액
  2. 식물추출물 및 in vitro 시험설계
  3. 조사 항목 및 조사 방법
  4. 통계처리
 Ⅲ. 결과 및 고찰
  1. 발효성상 및 미생물 성장량
  2. 휘발성지방산(VFA)
  3. 총 가스, 이산화탄소 및 메탄 발생량
  4. Real-Time PCR
 Ⅳ. 요약
 사사
 Ⅴ. REFERENCES

저자

• 이신자 [ Shin Ja Lee | 경상대학교 농업생명과학연구원&중점연구소 ]
• 이수경 [ Su Kyoung Lee | 경상대학교 농업생명과학연구원 ]
• 이상민 [ Sang Min Lee | ㈜이지바이오 ]
• 김도형 [ Do Hyung Kim | 경북도립대학교 축산과 ]
• 문여황 [ Yea Hwang Moon | 경남과학기술대학교 동물생명과학과 ]
• 이성실 [ Sung Sill Lee | 경상대학교 농업생명과학연구원&중점연구소 ] Corresponding author

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