Earticle

초록

영어

This study was conducted to evaluate effects of supplementation of Bifidobacterium ruminantium on in vitro ruminal fermentation and methane production. Ruminal fermentation characteristics of Timothy hay (C1), whole barley (C2), Timothy hay + B. ruminantium (T1) and whole barley + B. ruminantium (T2) were evaluated at 0, 3, 6, 9, 12, 24 and 48 h incubation at 39℃. The amount of B. ruminantium culture added into T1 and T2 was 0.3 ml. The pH values ranged from 5.99 to 6.83 in all the treatments. Concentration of NH3-N of C2 and T2 was higher than C1 and T1 at 48 h (p<0.05). The total gas production of C2 and T2 was higher than C1 and T1 at 9 h (p<0.05). The total methane production of treatments with B. ruminantium was not significantly different at 24 and 48 h (p<0.05). Concentration of lactic acid was significantly different between both substrates (p<0.05). Therefore, B. ruminantium supplementation was determined to be insignificant in the in vitro ruminal fermentation and methane production, while a further study was required to investigate relation to lactic acid production with different forage sources.

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 실험 재료
  2. 실험 동물 및 사양관리
  3. 배지 및 배양방법
  4. In vitro 반추위 발효실험
  5. 조사 항목 및 방법
  6. 통계처리
 Ⅲ. 결과 및 고찰
  1. In vitro 반추위 배양액 내 발효성상
  2. 총 가스 발생량 및 총 메탄 발생량
 Ⅳ. 요약
 사사
 Ⅴ. REFERENCES

저자

• 김세진 [ Se Jin Kim | 국립한경대학교 동물생명환경과학과 ]
• 최정호 [ Jung Ho Choi | 국립한경대학교 동물생명환경과학과 ]
• 김창현 [ Chang-Hyun Kim | 국립한경대학교 동물생명환경과학과 ] Corresponding author

참고문헌