Earticle

초록

영어

The objective of this study was to investigate the efficiency of nicotinic acid during in vitro fertilization (IVF) in frozen-thawed bull sperm . The ejaculated semen was diluted with Triladyl containing 20% egg-yolk and cryopreserved in liquid notrigen. The frozen sperm was thawed for 45 seconds in the 38℃ water bath. Sperm was diluted with IVF medium (Bovine-Oviduct medium; BO) containing 0, 15, 30 and 60 mM nicotinic acid (NA), which were incubated at 39℃, 5% CO2 for 0, 0.5, 1, 2 and 4h. The characteristics of frozen-thawed sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI for outer acrosomal membrane damage and Rhodamine123/PI for mitochondrial integrity using flow cytometry. And the sperm ability was analysed by Coomassie brilliant blue (CBB) staining for acrosome reaction state and Rose bengal staining for abnormality. Acrosome reaction and abnormality were analyzed using a microscope. In results, sperm viability was significantly higer in 30 mM group than 0 and 15 mM groups at 1 and 2 h (p<0.05). Outer acrosomal membrane damage was significantly lower in 30 mM group than 0 and 15 mM groups at 1, 2 and 4 h (p<0.05). And mitochondrial integrity was significantly higher in 30 mM group than 0 and 15 mM groups at 2 and 4 h (p<0.05). Also, acrosome reaction was significantly lower in 30 mM than 0 and 15 mM groups at 1 and 2 h (p<0.05) and abnormality was lower NA groups than 0 group at 1 h (p<0.05). In couclusion, we suggest that using the thawing medium containing NA for sperm dilution can be benefical for IVF in bulls.

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 정액의 채취 및 동결과 융해
  2. Bo-medium 준비 및 Nicotinic acid 첨가 후 시간별 배양
  3. 생존율, 첨체외막 손상율 및 미토콘드리아 온전성
  4. 첨체상태 및 기형율
  5. 통계분석
 Ⅲ. 결과
  1. 정자의 생존 능력
  2. 정자 첨체 외막 손상율
  3. 정자의 미토콘드리아 온전성
  4. 정자의 첨체반응 및 기형율
 Ⅳ. 고찰
 Ⅴ. 요약
 사사
 Ⅵ. REFERENCES

저자

• 김유진 [ Yu-Jin Kim | 강원대학교 동물생명과학대학 ]
• 이상희 [ Sang-Hee Lee | 강원대학교 동물생명과학대학 ]
• 양진우 [ Jin-Woo Yang | 강원대학교 동물생명과학대학 ]
• 이연주 [ Yeon-Ju Lee | 강원대학교 동물생명과학대학 ]
• 최수빈 [ Su-Bin Choi | 강원대학교 동물생명과학대학 ]
• 이경진 [ Kyung-Jin Lee | 강원대학교 동물생명과학대학 ]
• 이승형 [ Seunghyung Lee | 강원대학교 동물생명과학대학 ]
• 박춘근 [ Choon-Keun Park | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌