Earticle

초록

영어

This study was carried out to compare the anti-inflammation effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. We concentrated Ganoderma species and other medicinal mushrooms by extracting with ethanol. And We made it 100 μg/ml concentration. As a result of nitrite scavenging activity, in the contrast to the positive control; Ascorbic acid was 25%, ASI 7080 of Ganoderma species was disappeared up to around 40%. And in the contrast to Ascorbic acid was 55%, ASI 7002 was 78.5% that was the highest anti-inflammation effect in the result of “No assay test”. The Cordyceps militaris showed 75% and Hericium erinaceus showed 59.7% of anti-inflammation effect. As a result of the fungus yield control test of TNF-α through ELISA method to ASI 7002 of Ganoderma species that showed the highest anti-inflammation, it was reduced as same as LPS non-treatment. We extracted RNA from ASI 7002 Ganoderma species 10, 50, 100 μg/ml concentration and LPS 10 μg/ml of Raw 264.7 cell. And we tested the expression of iNOS, COX-2 and TNF-a that are kinds of inflammation gene after synthesizing RNA with cDNA. Finally we could find that iNOS, COX-2 and TNF-a were all controlled expression in the result of above experiment.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주
  아질산염(NaNo2) 소거능(Nitrite-scavenging activity)
  항염증 효과 (NO assay)
  Real-time PCR
  사이토카인류 TNF-α 분비량 측정 (ELISA : EnzymeLinked Immunosorbent assay)
 결과 및 고찰
  아질산염 소거능 (Nitrite-scavenging activity)
  함염증 효과 (NO assay)
  Real time PCR
  사이토카인류 TNF-α 분비량 측정 (ELISA : EnzymeLinked Immunosorbent assay)
 적요
 References

저자

• 조재한 [ Jae-Han Cho | 국립원예특작과학원 버섯과 ]
• 이강효 [ Gang-Hyo Lee | 국립원예특작과학원 버섯과 ]
• 한재구 [ Jae-Gu Han | 국립원예특작과학원 버섯과 ]
• 김형돈 [ Hyung-Don Kim | 국립원예특작과학원 인삼특작이용팀 ]
• 전창성 [ Chang-Sung Jhune | 월간버섯 J＆K 버섯연구소 ] Corresponding author

참고문헌