Earticle

초록

영어

In order to isolate compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated CA105 was selected by agar diffusion method. The strain CA105 was identified as members of the Bacillus subtilis by biochemical characteristics using VITEK 2 system. Comparative 16S rRNA gene sequence analysis showed that isolate CA105 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rRNA gene sequence similarity of 98.9%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, isolate CA105 was classified within the genus Bacillus subtilis, for which the name Bacillus subtilis CA105 is proposed. The cellulase and xylanase activity of B. subtilis CA105 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. CA105.

목차

ABSTRACT
 서론
 재료 및 방법
  느타리버섯 수확후배지에 우점하는 미생물 분리
  분리균의 생리적 특성 및 생화학적 특성 조사
  분리균의 16S rRNA 염기서열 분석
  분리균이 분비하는 가수분해효소
  분리균의 생육과 효소 생성능
 결과 및 고찰
  느타리버섯 수확후배지에 우점하는 CA105 균주의 분리
  분리균 CA105의 동정
  분리균 CA105 균주가 분비하는 cellulase와 xylanase의특성
 적요
 참고문헌

저자

• 김혜수 [ Hye Soo Kim | 경남과학기술대학교 제약공학과 ]
• 김철환 [ Chul Hwan Kim | 경남과학기술대학교 제약공학과 ]
• 권현숙 [ Hyun Sook Kwon | 한국한방산업진흥원 ]
• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ] Corresponding author

참고문헌