Earticle

초록

영어

Hypsizigus marmoreus is commercially the most important edible mushroom in Japan. This mushroom is usually cultivated for a longer period (about 85~120 days) than other mushroom. In order to develop a new cultivar that has a shortened cultivation period, the genome analysis of this strain has been considered. This study aims to obtain parental monokaryotic strains reproducing ‘Haemi’ cultivar in Hypsizigus marmoreus for reference genome sequencing. The mycelia were cultured in MCM and MYG media for various incubation periods. Homogenized mycelia were treated with commercial cell wall degrading enzymes to maximize protoplasts production yield from Hypsizigus marmoreus. The greatest number of protoplasts was obtained from mycelia cultured in MCM media for 3 days using Novozyme enzyme. The isolated protoplasts were grown in regeneration agar media after two weeks. Regenerated colonies were picked and moved on separated dishes for microscopic observation. Neohaplonts regenerated from dikayotic strains were identified by the absence of clamp connections. We confirmed that one of monokaryotic strains is a parental strain by crossing with an original compatible strain of ‘Haemi’ cultivar. This parental strain will be used for reference genome sequence analysis.

목차

ABSTRACT:
 서론
  원형질체 분리조건 및 재생조건 확립
  모균주의 단핵균주 분리
 적요
 감사의 말씀
 References

저자

• 오연이 [ Youn-Lee Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab-Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 김은선 [ Eun-Sun Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오민지 [ Min ji Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 최인걸 [ In-Geol Choi | 고려대학교 생명공학과 대학원 생명공학과&BK21플러스 ] Corresponding author

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