Earticle

초록

영어

Agaricus bitorquis is an edible white mushroom of the genus that is cultivated at high temperature (25±1℃) unlike A. bisporus is cultivate at 16±2℃. Unlike Agaricus bisporus, an edible white A. bitorquis mushroom is cultivated at high temperature (25±1℃). Most farmers cultivate this mushroom for a long cultivation period in Korea. For this reason, we made heterokayons to develop a new cultivar that generate fruitbodies for short cultivation period. Over one hundred SSIs(single spores isolates) were collected from selected A. bitorquis ASI1151 and ASI1349 strains. Seventy-three SSIs were germinated on CDA(compost dextrose agar) media after 20 days (minimum) or 83 days (maximum) incubation under different media condition. The mycelial growth rate of germinated SSIs was different. 9 homokaryons in ASI 1151 and 11 homokaryons in ASI 1349 from SSIs were selected by OPN-02 primer in RAPD analaysis. Also this primer was used to select heterokaryon that cross among each homokaryon with compatible locus. Therefore 44 compatible matings were confirmed of 99 crossed lines.

목차

ABSTRACT:
 서론
 재료 및 방법
  모본 선발 및 단포자 분리
  단포자 분리
  동핵균주 분리를 위한 RAPD분석
  교잡 및 교잡 확인
 결과 및 고찰
  모본 선발 및 단포자 분리
  동핵균주 분리 및 교잡주확인을 위한 RAPD분석
 적요
 감사의 말씀
 References

저자

• 오연이 [ Youn-Lee Oh | 고려대학교 생명공학과 대학원 생명공학과&BK21플러스 ]
• 장갑열 [ Kab-Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 김은선 [ Eun-Sun Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오민지 [ Min ji Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 최인걸 [ In-Geol Choi | 고려대학교 생명공학과 대학원 생명공학과&BK21플러스 ] Corresponding author

참고문헌