Earticle

초록

영어

Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCR polymorphism could potentially be used to identify homokaryon isolates.

목차

ABSTRACT:
 서론
 재료 및 방법
  공시균주 및 배양
  Genomic DNA 추출
  PCR 다형성 분석
  유전적 유연관계 분석
 결과 및 고찰
  양송이 계통간 ISSR-PCR 다형성 및 유연관계분석
  ISSR 마커에의한 단포자 PCR다형성 분석
 적요
 감사의 글
 References

저자

• 민경진 [ Kyong-Jin Min | 한경대학교 미래융합기술대학원, (주)제이케이바이오테크 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립특작과학원 버섯과 ]
• 강희완 [ Hee-Wan Kang | 한경대학교 미래융합기술대학원, (주)제이케이바이오테크, 한경대학교 유전공학연구소 ] Corresponding author

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