Earticle

초록

영어

Hybrid strains was selected by crossing between dikaryotic strain ASI 0628 (Dagul) bred from ASI 2596 (Suhan No.3) and ASI 2782(Black pileus mutant) and monokaryotic strain ASI 2344-84. The strain 84 that shown the best cultural characteristics was selected to be a new cytoplasmic hybrid variety and named as ‘Chunhwashim’. The ‘Chunhwashim’ shown incompatibility among them forming the confrontation growth line against parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho). Fruiting body produced about 161.4±4.8 g per bottle. And also the individual generation of fruit body is multiple than chunchu2ho as 40.9. The ‘Chunhwashim’ was cytoplasmic hybrid included hybrid DNA bands of parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho) by Random Amplified Polymorphic DNA (RAPD) primer, and mitochondria DNA band of monokaryotic ASI 2344 (chunchu 2ho) using mitochondria microsatellite DNA marker. This new cytoplasmic hybrid variety 'Chunhwashim' of oyster mushroom is characterized by multiple of individual generation and deeply grey color of pileus.

목차

서 론
 재료 및 방법
  시험균주 배양
  단포자 분리 및 교잡
  분자마커를 이용한 세포질전환 교잡주의 선발방법
  교잡계통 선발을 위한 자실체 특성 분석
  DNA 다형성 분석
 결과 및 고찰
  육성경위
  세포질전환 교잡주의 선발
  신품종 ‘천화심’의 주요특성
 적 요
 감사의 말씀
 References

저자

• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author
• 유영복 [ Young-Bok Yoo | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오연이 [ Youn-Lee Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]

참고문헌