Earticle

초록

영어

This study was conducted to investigate optimum conditions for mass production of antagonistic microbes Pseudomonas azotoformans HC5. P. azotoformans HC5 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This markedly showed the antagonistic activity against P. tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the P. azotoformans HC5, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 6.0 and 15oC, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium was determined as follows: 0.6% adonitol, 1.5% yeast extract, 0.8% NH4H2PO4, 5mM MgSO4, and 0.2% asparagine.

목차

서 론
 재료 및 방법
  공시균주 및 배양
  배양적온
  초기 pH의 영향
  영양원에 따른 길항미생물의 증식에 미치는 영향
 결과 및 고찰
  온도와 pH에 따른 영향
  탄소원에 따른 영향
  무기질소원에 따른 영향
  유기질소원에 따른 영향
  아미노산에 따른 영향
  무기염류에 따른 영향
  길항미생물 HC5의 최적 배양조건
 적 요
 감사의 말씀
 References

저자

• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author
• 문지원 [ Ji-Won Moon | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 유영미 [ Young-Mi Yoo | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 한주연 [ Ju-Yeon Han | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 정종천 [ Jong-Chun Cheong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]

참고문헌