Earticle

초록

영어

The insulin-like growth factor binding proteins (IGFBPs) is involved in the regulating effects and the biological activities of the insulin-like growth factors (IGFs). Recently, It has been reported that the IGFBP2 gene polymorphism was associated growth and skeletal development in chicken. This study was designed to investigate the association of the C1032T SNP of IGFBP2 gene with growth and egg production traits in Korean native chicken (KNC) population as well as other chicken breeds. A total of 225 chickens from the three breeds (Rhode Island Red, Cornish, Leghorn) and 245 chickens from the six strains of Korean native chickens including Black, Gray, Ogol, Red, White, and Yellow, were genotyped by PCR-RFLP (polymerase chain reaction-restricted fragment length polymorphism) and analyzed the association between their genotypes and economic traits by SAS program. The results showed that the SNP (single nucleotide polymorphism) was significantly associated with multiple traits, including body weight of 150 days (p<0.001 and p<0.05) in the Rhode Island Red and the KNC (White) and egg production number (p<0.05) in the KNC (Black) breed. These results suggest that IGFBP2 C1032T SNP could be used as a DNA marker for the improvement of economic traits in Korean native chicken breeding.

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 공시재료 및 DNA추출
  2. DNA추출
  3. PCR-RFLP를 이용한 IGFBP2 유전자 SNP의 유전자형 분석
  4. 통계분석
 Ⅲ. 결과 및 고찰
 Ⅳ. 요약
 Ⅴ. REFERENCES

저자

• 최소영 [ So-Young Choi | 강원대학교 동물생명과학대학 ]
• 원종영 [ Jong-Young Won | 강원대학교 동물생명과학대학 ]
• 김종대 [ Chong-Dae Kim | 농촌진흥청 국립축산과학원 ]
• 홍영호 [ Yeong Ho Hong | 중앙대학교 동물생명공학과 ]
• 정동기 [ Dong-Kee Jeong | 제주대학교 생명과학부 ]
• 이성진 [ Sung-Jin Lee | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌