Earticle

초록

영어

A lipase producing bacterium was isolated from button mushroom bed, which showing high clear zone on agar media containing Tributyrin as the substrate. The strain was identified as Burkholderia cepacia by analysis of 16S rDNA gene sequence. Crude lipase (CL) was partially purified from 70% ammonium sulfate precipitation using the culture filtrate of B. cepacia. Immobilized lipases were prepared by cross-linking method with CL from B. cepacia and Novozyme lipase (NL) onto silanized Silica-gel as support. Residual activitiy of the immobilized CL (ICL) and immobilized NL (INL) was maintained upto 61% and 72%, respectively. Biodiesel (Fatty acid methyl ester, FAME) was recovered by transesterification and methanolysis of Canola oil using NaOH, CL and ICL as the catalysts to compare the composition of fatty acids and the yield of FAME. Total FAME content was NaOH 781 mg L-1, CL 681 mg L-1 and ICL 596 mg L-1, in which the highest levels of FAME was observed to 50% oleic acid (C18:1) and 22% stearic acid (C18:0). In addition, the unsaturated FAME (C18:1, C18:2) decreased, while saturated FAME (C16:0, C18:0) increased according to increasing the reaction times with both CL and ICL, supporting CL possess both transesterification and interesterification activity. When reusability of ICL and INL was estimated by using the continuous reaction of 4 cycles, the activity of ICL and INL was respectively maintained 66% and 79% until the fourth reaction.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  Lipase 생산균의 분리 및 동정
  Lipase 조효소 제조
  Lipase 고정화
  Lipase Activity 측정 및 Lowry method
  Biodiesel 제조 및 분석
  화학적 촉매, 효소적 및 고정화효소 촉매법
  Free lipase를 이용한 interesterification 반응
  통계처리
 결과 및 고찰
  Lipase 생산균의 분리 및 lipase 조효소 제조
  Lipase 고정화
  촉매에 따른 바이오디젤 전환
  효소촉매 반응시간에 따른 바이오디젤 전환
  Lipase를 이용한 interesterification 반응물의 지방산 및 TAG 조성
  Immobilized lipase의 재사용성(reusability)
 적요
 감사의 글
 References

저자

• 김현희 [ Heon-Hee Kim | 충남대학교 농업생명과학대학 생물환경화학과 ]
• 김찬겸 [ Chan-Kyum Kim | 충남대학교 농업생명과학대학 생물환경화학과 ]
• 한창훈 [ Chang-Hoon Han | 충남대학교 농업생명과학대학 생물환경화학과 ]
• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 윤민호 [ Min-Ho Yoon | 충남대학교 농업생명과학대학 생물환경화학과 ] Corresponding author

참고문헌