Earticle

초록

영어

Our study investigated the fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of the water extract and solvent fractions isolated from Pleurotus ferulea. Fibrinolytic activity was investigated using the fibrin plate method. Thrombin inhibitory activity was used to analyze thrombin inhibitor assay. The DPPH assay was used to estimate antioxidative activity. Inhibition of NO production was measured for anti-inflammatory activity in LPS-activated murine RAW 264.7 macrophage cells. An MTS assay was used to evaluate the effects of the water extract and solvent fractions isolated from Pleurotus ferulea on cell viability. Our results showed the fibrinolytic activity to be strong in the ethyl acetate fraction at 1.33 plasmin units. The ethyl acetate fraction also showed high thrombin inhibitory activity at 94.45%. The anti-oxidative activity of the water extract was 37.01% and the anti-inflammatory activity of the chloroform fraction was 98.13%. These findings suggest that Pleurotus ferulea's extract and fractions could be applicable in the development of functional foods for the treatment and prevention of cardiovascular diseases.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  세포배양
  물 추출물과 유기용매 분획물 조제
  혈전용해활성 측정
  트롬빈 저해활성 측정
  전자공여능 측정
  세포독성 측정
  NO 생성 저해 효과 측정
  통계 분석
 결과 및 고찰
  용매별 분획물의 수율
  혈전용해활성
  트롬빈 저해활성
  항산화효과
  세포독성 조사
  Nitric Oxide (NO) 생성 억제 효과
 적요
 References

저자

• 김은정 [ Eun-Jung Kim | 상지대학교 임상병리학과 ]
• 김준호 [ Jun-Ho Kim | 상지대학교 정밀화학신소재학과 ] Corresponding author

참고문헌