There is cumulative evidence that the Akt and Ras are involved in neuronal survival and protection. The Akt and Ras activates, resulting in the regulation of survival proteins such as BAD and Bcl-xL. Thus, Akt/Ras signaling pathway may be one propitious target for treatment of neurological disease. Pulsed electromagnetic field (PEMF) is known to have effects on biological properties, such as differentiation, regulation of transcription factor and cell proliferation. However, the cell protective effect of PEMF exposure is largely unknown. The aim of this study is to understand the underlying mechanisms that PEMF suppressing apoptosis and promoting survival, including PEMF induced neuronal differentiation. In induced human BM-MSCs treated with PEMF, the expression of neural msarkers such as NF-L, NeuroD1 and Tau increased. Moreover, treatment of induced human BM-MSCs with PEMF exposure greatly decreased cell death in the dose and time dependent manner. Additionally, Analysis indicated that PEMF exposure dramatically promoted activities of Akt, Rsk, Creb, Erk, Bcl-xL and Bad phosphorylation. Meanwhile, the PEMF dependent cell protection was notably reversed by pretreatment with LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K). Our data suggest that PI3K/Akt/Bad signalling pathway may be a possible mechanism involved in the cell protective effect of PEMF.
저자
Enerelt Urnukhsaikhan [ Medical Biotechnology, Dongguk University Doctoral student at Dongguk University ]
