Earticle

초록

영어

Coprinellus micaceus, belongs to family Psathyrellaceae of Agaricales, Basidiomycota, has been used for edible purposes in the world. This study was initiated to evaluate the antioxidant, anti-diabetic, anti-cholinesterase, anti-tyrosinase, and nitric oxide inhibitory activities of fruiting bodies from C. micaceus extracted with methanol and hot water. The HPLC analysis of phenolic compounds from the mushroom extracts identified 4 phenolic compounds including procatechuic acid, chlorogenic acid, (-)-epicatechin, and naringin. In 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging assay, the scavenging activities of methanol and hot water extracts were lower than that of positive control, BHT. The chelating effects of methanol and hot water extracts were significantly higher than that of BHT, the positive control at the all concentrations tested. In the reducing power assay, methanol and hot water extracts exhibited the lower activities compared with positive control at the 0.125-0.2 mg/ ml. The methanol and hot water extracts of the mushroom inhibited the α -glucosidase activity by 62.26% and 67.59%, respectively at the 2.0 mg/ml, while acarbose, the positive control, inhibited the α-glucosidase activity by 81.81% at the same concentration. In the acetylcholinesterase(AChE) inhibitory activity assay, methanol and hot water extracts of the mushroom inhibited the AChE by 94.64% and 74.19%, respectively at 1.0 mg/ml, whereas the galanthamine, standard drug, inhibited the AChE activity by 97.80% at the same concentration. The tyrosinase inhibitory activities of methanol and hot water extracts were 91.33% and 91.99% at 2.0 mg/ml, while the inhibitory activity of kojic acid, the positive control, was 99.61% at the same concentration. Nitric oxide(NO) production in lipopolysaccahride (LPS) activated RAW 264.7 cells were inhibited by the methanol and hot water extracts in a concentration dependent manner. Therefore, it is concluded that fruiting bodies of C. micaceus contained natural antioxidant, anti-acetylcholinesterase and α- glucosidase inhibitory, anti-inflammatory, anti-tyrosinase substances which might be used for promoting human health.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  성분의 추출 및 분리
  페놀성 화합물의 분석
  세포배양
  추출물의 세포 독성 측정
  DPPH 라디칼 소거 활성
  철 이온 제거능
  환원력의 측정
  α-amylase 저해 활성 측정
  α-glucosidase 저해 활성 측정
  Acetylcholinestrase (AChE) 저해 활성 측정
  Butyrylcholinestrase (BChE) 저해 활성 측정
  티로시나아제 저해 효과 측정
  Nitric oxide(NO) 생성 저해효과 측정
  통계 처리
 결과 및 고찰
  페놀성 화합물의 분석
  세포독성
  DPPH 라디칼 소거능
  철 이온 제거능
  환원력
  α-amylase 저해 활성
  α-glucosidase 저해 활성
  Acetylcholinestrase (AChE) 저해 활성
  Butyrylcholinestrase (BChE) 저해 활성
  티로시나아제 저해 효과
  Nitric oxide(NO) 생성 저해 효과
 적요
 References

저자

• Trung Kien Nguyen [ 인천대학교 생명과학부 ]
• 이민웅 [ Min Woong Lee | 동국대학교 생명과학과 ]
• 윤기남 [ Ki Nam Yoon | 안산대학교 임상병리과 ]
• 김혜영 [ Hye Young Kim | 신성대학교 임상병리과 ]
• 진가헌 [ Ga-Heon Jin | 신한대학교 안경과학과 ]
• 최재혁 [ Jae-Hyuk Choi | 인천대학교 생명과학부 ]
• 임경환 [ Kyung Hoan Im | 인천대학교 생명과학부 ]
• 이태수 [ Tae Soo Lee | 인천대학교 생명과학부 ] Corresponding author

참고문헌