Earticle

초록

영어

In this study, SCAR marker that differentiates Pleurotus eryngii strains adaptable to high-temperature from control strain was developed. Genomic DNAs of 7 control strains of Pleurotus eryngii and 7 Pleurotus eryngii strains adaptable to hightemperature were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). Onehundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 385 bp was yielded by OP-A06 primer from the Pleurotus eryngii strains adaptable to high- temperature. A sequence characterized amplified region (SCAR) marker, designated as OP-A06-1-F and OP-A06-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-A06-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains adaptable to high-temperature from the control strains.

목차

ABSTRACT
 서론
  대립 계통과 고온성 계통 선발
  RAPD를 이용한 bulked segregant analysis
  SCAR 마커 디자인
 적요
 감사의 글
 참고문헌

저자

• 김수철 [ Su Cheol Kim | 경남과학기술대학교 제약공학과 ]
• 김혜수 [ Hye Soo Kim | 경남과학기술대학교 제약공학과 ]
• 박소연 [ So Yeon Park | 경남과학기술대학교 제약공학과 ]
• 류재산 [ Jae-San Ryu | 경상남도농업기술원 친환경연구과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ] Corresponding author

참고문헌