Earticle

초록

영어

Oyster mushrooms including of P. ostreatus, P. eryngii, P. pulmonarius and P. cornucopiae are one of the famous mushrooms for foods in Korea. RAPD were carried out using 14 of oligoprimers to analyze the phylogenetic relationship among 57 strains of 32 Pleurotus species. Most of species formed the minimum clade with strains within species and was divided respectively species. Therefore clade was separated well in accordance species. Pleurotus species formed again clade to be added in close related to other species, and were discriminated by sixteen clades with each representative species including high similarity groups. Sixteen clades were composed representative species according to each clade. There were clade I of P. pulmonarius(P. sajor-caju, P. opuntiae, P. sapidus), clade II of P. eryngii(P. fuscus var. ferulae, P. fossulatus), clade III of P. ostreatus(P. ostreatus var. columbinus, P. spodoleucus, P. floridanus), clade IV of P. florida, clade V of P. djamor(P. flabellatus, P. incarnates, P. salmoneo-stramineus), clade VII of P. populinus(P. subareolatus), clade VIII of P. cystidiosus(P. cystidiosus var. formosensis), clade X of P. dryinus(P. dryinus var. pometi), clade XIV of P. cornucopiae(P. citrinopilieatus, P. euosmus), and clade XV of P. australis. These species were representative species each clades. Five species, P. ulmarius(clade VI), P. griseus(clade IX), P. calyptratus(clade XI), P. lampas(clade XII), P. smithii(clade XIII)and P. serotinus (clade XVI) were used each one strain in analysis, so they were clustered other groups.

목차

ABSTRACT
 Introduction
 Material and Methods
  Fungal Strains
  Sample Preparation and Genomic DNA Extraction
  Amplification of RAPD using arbitrary primers
  Phylogenetic analysis of DNA amplification fingerprints
 Results and Discussion
 Acknowledgements
 References

저자

• Sun-Gyu Choi [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Republic of Korea ]
• Kab-Yeul Jang [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Republic of Korea ]
• Gyu-Hyun Kim [ Department of Biotechnology, Yonam college of Agriculture, Chungnam, 360-763, Republic of Korea ]
• Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Republic of Korea ]
• Jae-Sun Jo [ Department of Food Engineering, Kyung Hee University, Suwon, 449-701, Republic of Korea ]
• Hae-Yeong Kim [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Republic of Korea ]
• Young-Bok Yoo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Republic of Korea ] Corresponding author

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