Earticle

초록

영어

Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were 25oC and 30oC. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

목차

ABSTRACT
 Introduction
 Material and Methods
  Fungal Strains
  Media
  Temperature
  pH
  Sample preparation and genomic DNA extraction
  PCR amplification of IGS I region in rDNA
  Restriction enzyme digestion and electrophoresis
  Phylogenetic analysis
 Results and Discussion
  Cultural Characteristics
  Phylogenetic relationships based on RFLP of IGS I region in rDNA
 Acknowledgements
 References

저자

• Sun-Gyu Choi [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Korea ]
• Kab-Yeul Jang [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Korea ]
• Gyu-Hyun Kim [ Dep. of Biotechnology, Cheonan Yonam College, Chungnam, 360-763, Republic of Korea ]
• Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Korea ]
• Jae-Sun Jo [ Dep. of Food Science and Biotechnology, Kyung Hee University, Suwon, 449-701, Republic of Korea ]
• Hae-Yeong Kim [ Dep. of Food Science and Biotechnology, Kyung Hee University, Suwon, 449-701, Republic of Korea ]
• Young-Bok Yoo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA Chungbuk Eumseong 369-873, Korea ] Corresponding author

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