Earticle

초록

영어

Dictyophora indusiata is an edible mushroom belongs to Family Phallaceae of Phallales, Basidiomycota. The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of methanol and hot water extracts prepared from fruiting bodies of Dictyophora indusiata. Besides measuring of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, a reducing power and a chelating activity on ferrous ions were also measured to evaluate the antioxidant activity for those extracts. To measure the anti- inflammatory activities for the extracts, nitric oxide(NO) production from lipopolysaccharide(LPS) treated RAW 264.7 macrophage cells and carrageenan-induced acute hind paw edema of rats were investigated. The results showed that the extracts have excellent DPPH scavenging and chelating activity on the ferrous ions compared with positive control. The nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophage cells were decreased as we increased the concentration of the mushroom extracts. Significant reduction of paw edema of rats were observed at 2~6 h after treatment of methanol and hot-water extracts with 50 mg/ kg concentration to the rats which are induced acute hind paw edema by carrageenan administration. Therefore, the experimental results suggested that methanol and hot-water extracts of Dictyophora indusiata fruiting bodies might be used for natural sources of antioxidant and anti-inflammatory agents.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  성분의 추출 및 분리
  총 폴리페놀 함량 측정
  총 플라보노이드 함량 측정
  세포배양
  세포의 생존율 측정
  DPPH 라디칼 소거 활성 측정
  환원력 활성 측정
  철 이온 제거능
  Nitrite(NO) 생성 저해효과 측정
  Western blot analysis
  Carrageenan에 의한 부종유발 및 소염효과의 측정
  통계 처리
 결과 및 고찰
  총 폴리페놀 및 플라보노이드 함량
  세포독성 분석
  DPPH 라디칼 소거활성
  철 이온 제거능
  환원력
  NO 생성 저해 효과
  Western blot analysis
  Carrageenan에 의한 부종유발 및 소염효과의 측정
 적요
 감사의 말씀
 참고문헌

저자

• Trung Kien Nguyen [ 인천대학교 생명과학기술대학 생명과학부 ]
• 신도빈 [ Do Bin Shin | 인천대학교 생명과학기술대학 생명과학부 ]
• 이경림 [ Kyung Rim Lee | 인천대학교 생명과학기술대학 생명과학부 ]
• 신평균 [ Pyung Gyun Shin | 원예특작과학원 버섯과 ]
• 정종천 [ Jong Chun Cheong | 원예특작과학원 버섯과 ]
• 유영복 [ Young Bok Yoo | 원예특작과학원 버섯과 ]
• 이민웅 [ Min Woong Lee | 동국대학교 ]
• 진가헌 [ Ga-Heon Jin | 신흥대학교 안경과학과 ]
• 김혜영 [ Hye Young Kim | 신성대학교 임상병리과 ]
• 임경환 [ Kyung Hoan Im | 인천대학교 생명과학기술대학 생명과학부 ]
• 이태수 [ Tae Soo Lee | 인천대학교 생명과학기술대학 생명과학부 ] Corresponding author

참고문헌