Earticle

초록

영어

The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanol extracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) and extracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometric methods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidant activities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanol extract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and 5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and 1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinase inhibitory activity of the methanol extract (200 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than those of positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus and stipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6 mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2- (4-nitrophenyl)-2H-5-tetrazolio]-1,3- benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higher proliferation rate than those of 0.04% adenosine.

목차

ABSTRACT
 I. 서론
 II. 재료 및 방법
  갈색 느티만가닥버섯 메탄올 추출물의 제조
  총 폴리페놀 함량 측정
  총 플라보노이드 함량 측정
  Tyrosinase 저해 활성
  DPPH 라디칼소거 활성
  B16BL6 세포 배양
  세포 생존율 측정
  통계처리
 결과 및 고찰
  갈색 느티만가닥버섯 메탄올 추출물의 총 폴리페놀및 플라보노이드 함량
  갈색 느티만가닥버섯 메탄올 추출물의 tyrosinase 저해 활성
  갈색 느티만가닥버섯 메탄올 추출물의 DPPH 라디칼소거 활성
  갈색 느티만가닥버섯 메탄올 추출물이 B16BL6 mouse melanoma cell에 미치는 영향
 적요
 감사의 말씀
 참고문헌

저자

• 김수철 [ Su Cheol Kim | 경남과학기술대학교 제약공학과 ]
• 류한민 [ Han Min Ryu | 경남과학기술대학교 제약공학과 ]
• 정성미 [ Sung Mi Jung | 경남과학기술대학교 제약공학과 ]
• 이용현 [ Young Hyun Lee | 경남과학기술대학교 제약공학과 ]
• 김혜수 [ Hye Soo Kim | 경남과학기술대학교 제약공학과 ]
• 김종옥 [ Jong Ok Kim | 남경농산 ]
• 조용운 [ Young Un Cho | 경남과학기술대학교 제약공학과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ] Corresponding author

참고문헌