Earticle

초록

영어

Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were 20oC and pH 4, while optimal conditions for mycelial growth of A. mellea were 25oC and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at 20oC under dark condition.

목차

ABSTRACT
 Introduction
 Materials and Methods
  Fungal strains
  Culture conditions for mycelial growth of P.
  Effect of nutrient source
  Effect of nutrient source for sclerotium formation
  Statistical analysis
 Results and Discussion
  Culture conditions for mycelial growth of P.
  Nitrogen sources
  Effect of nutrient sources and pH values for sclerotium formation
 Acknowledgement

저자

• Min Woong Lee [ Department of Life Science, Dongguk University, Seoul 100-715, Korea ]
• Kwang Chun Chang [ Department of Life Science, Dongguk University, Seoul 100-715, Korea ]
• Do Bin Shin [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
• Kyung Rim Lee [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
• Kyung Hoan Im [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ]
• Ga-Heon Jin [ Department of Ophthalmic Optics, Shinheung University 95 Hoam-ro, Uijeongbu, Gyeongggi 480-701, Korea ]
• Pyung Gyun Shin [ Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Korea ]
• Yong Mei Xing [ of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, eijing 100094, People's Republic of China. ]
• Juan Chen [ Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, People's Republic of China. ]
• Shun Xing Guo [ Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, People's Republic of China. ]
• Tae Soo Lee [ Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea ] Corresponding author

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