Earticle

초록

영어

Selection based on phenotype in the traditional manner does not help in tracking the selected gene. Molecular genetics has revolutionized the old established breeding techniques. A new epoch of molecular markers has been acquainted for genetic improvement of livestock. This study is engaged on the neoteric molecular markers used in various fields of livestock. DNA markers are more encouraging in selecting genomes that have recombination events close to the target gene. Molecular markers rely on DNA assay and are better than the morphological and biochemical markers. In this study DNA-based molecular markers developed during the past decagons for animal genome analysis are reviewed. Mapping of molecular markers provide a framework, required for its subsequent use in the selection procedure. Molecular techniques help in the utilization of genetic variability in breeding population. At the same time livestock genomes play important role in human genomics and their role for understanding human genomics cannot be overlooked. Recently, in the epigenetic and transcriptomic studies, RNA sequencing as a part of next generation sequencing has revolutionized the approach and ongoing trends of analysis. Keeping in mind the goals, these molecular techniques can be implemented successfully by following well defined, crisp and integrated strategy.

목차

ABSTRACT
 INTRODUCTION
 MOLECULAR MARKERS (MMs)
 RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs)
 PCR-BASED DNA MARKERS
 RANDOM AMPLIFIED LENGTH POLYMORPHIC DNAs(RAPDs)
 AMPLIFIED FRAGMENT LENGTH POLYMORPHISM(AFLP)
 MICROSATELLITES (MS)
 QUANTITATIVE TRAIT LOCI (QTL)
 CANDIDATE GENES
 MARKER ASSISTED SELECTION (MAS)
 SINGLE NUCLEOTIDE POLYMORPHISMS (SNP)
 LIVESTOCK GENOME PROJECTS
 NEXT GENERATION SEQUENCING (NGS)
 CHALLENGES TO FACE
  Low input systems
  Changes in breeding programmes
  Publically funded genomic research
 CONCLUSION
 ACKNOWLEDGEMENT
 REFERENCES

저자

• Simrinder Singh Sodhi [ 제주대학교 생명공학부 동물생명공학전공 ]
• Neelesh Sharma [ 제주대학교 생명공학부 동물생명공학전공 ]
• 오성종 [ Sung Jong Oh | 제주대학교 생명공학부 동물생명공학전공 ]
• 정동기 [ DongKee Jeong | 제주대학교 생명공학부 동물생명공학전공 ] Corresponding Author

참고문헌