Earticle

초록

영어

Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti- inflammatory, and melanin synthesis inhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extracts showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions compared with the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS- induced RAW 264.7 macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h after administration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg of indomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with 100 μg/mL of the methanol and hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, the experimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used for good sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료
  성분의 추출 및 분리
  총 폴리페놀 함량 측정
  총 플라보노이드 함량 측정
  DPPH 라디칼 소거 활성 측정
  철 이온 제거능 측정
  환원력 활성 측정
  추출물의 세포 독성 측정
  Nitrite (NO) 생성 저해효과 측정
  Carrageenan에 의한 흰쥐 뒷발의 부종유발 및 소염효과의 측정
  티로시나아제 활성 저해능 측정
  Melanoma B16/F10 세포내 멜라닌의 생성억제 측정
  통계 처리
 결과 및 고찰
  총 폴리페놀 및 플라보노이드의 함량
  DPPH 라디칼 소거활성
  철 이온 제거능
  환원력
  추출물의 세포독성
  NO 생성 저해
  Carrageenan에 의한 부종유발 및 항염 효과
  티로시나아제 활성 저해
  Melanoma B16/F10 세포내 멜라닌의 생성 저해
 적요
 감사의 글
 참고문헌

저자

• 권예주 [ Ye Ju Kwon | 인천논현고등학교 ]
• 김미현 [ Mi-Hyeon Kim | 인천논현고등학교 ]
• 최재순 [ Jae Soon Choi | 인천논현고등학교 ]
• 이태수 [ Tae Soo Lee | 인천대학교 생명과학기술대학, 생명과학부 ] Corresponding author

참고문헌