버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소 (tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건
Cultivation conditions for mass production of detoxifying bacterium Pseudomonas sp. HC1 of tolaasin produced by Pseudomonas tolaasii
Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and 20oC, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% (NH4)2HPO4, 4mM FeCl3, and 3.0% cysteine.
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ABSTRACTS 서론 재료 및 방법 공시균주 및 배양 배양적온 초기 pH의 영향 영양원에 따른 독소저해균의 증식에 미치는 영향 결과 및 고찰 온도와 pH에 따른 영향 탄소원에 따른 영향 무기질소원에 따른 영양 유기질소원에 따른 영향 아미노산에 따른 영향 무기염류에 따른 영향 적요 감사의 말씀 참고문헌