Earticle

초록

영어

Mycelial viability and cultivation characteristics of strains of Hypsizygus marmoreus after long-term storage were investigated. The experimental conditions were the storage at 4oC using a slant culture technique with or without mineral oil, and in liquid nitrogen tank in the presence of 10% glycerol or 10% glycerol with 5% trehalose. The myceila of four strains of H. marmoreus were thawed at 9, 21, 33, and 45 months after beginning of the storage, and then the growth of the mycelia was measured on a PDA plate with serial transfers to new plate for the recovery. The mycelial growth data after 45 months showed that the mycelia were mostly viable but not fully active particularly when they were stored in liquid nitrogen with 10% glycerol. The growth activity could be fully recovered after second transfer to new PDA plate. Cultivation of mushroom fruiting body using the recovered mycelia also demonstrated that the storage methods employed in this work were applicable for the long-term storage of H. marmoreus.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주
  계대배양법
  사면배지 보존법
  Mineral oil 보존법
  액체질소 보존법
  균주 생존 능력 측정
  균주 재배 성능의 측정
 결과 및 고찰
  장기 저장에 따른 균주의 생존력 검증
  장기 저장에 따른 균주의 재배성능 검증
 적요
 감사의 글
 참고문헌

저자

• 이혜정 [ Hyae-Jung Lee | 그린피스 버섯연구소 ]
• 노현수 [ Hyeon-Su Ro | 경상대학교 미생물학과 ]
• 김종국 [ Jong-Guk Kim | 경북대학교 생명과학부 ]
• 이창윤 [ Chang-Yun Lee | 경북대학교 생명과학부 ] Corresponding author

참고문헌