We have used bulked segregant analysis to screen the strain-specific DNA marker associated psychrophilic strain of Pleurotus eryngii. Bulked genomic DNAs of Pleurotus eryngii were amplified by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S primers to screen the strain-specific DNA marker. A unique DNA fragment of 490 bp was amplified with OP-L18 primer from the psychrophilic strain and sequenced. A sequence characterized amplified region (SCAR) marker was designed on the basis of the determined sequence and named as OP-L18-1. The PCR analysis with the OP-L18-1 primer showed that this SCAR marker clearly distinguish the psychrophilic strains from the control strains.
저자
Hong Chul Kim [ Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea, ]
YunJinCho [ Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea, ]
Su Cheol Kim [ Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea, ]
Hye Sung Hwang [ Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea, ]
Jae-San Ryu [ Eco-friendliness Research Department , Gyeongnam-doAgricultural Rearch and Extension Services, Jinju 660-360, Korea ]
Soo Jeong Cho [ Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea, ]
