Flammulina velutipes is a popular edible basidiomycete mushroom found in East Asia and is commonly known as winter mushroom. Mushroom development showing dramatic morphological changes by different environmental factors is scientifically and commercially interesting. We have sequenced the 35.6-megabase pair genome of F. velutipes KACC42780 using a next generation sequencing (NGS) approach. Then, HiSeq2000 sequencing system was used for comparing gene expression of F. velutipes genome at three different developmental stages (mycelium, primordium, and fruit body). Ninety one percents (10,116 genes) of the 10,999 predicted genes are expressed in at least one developmental stage. In addition, analysis of expressed genes in three different developmental stages showed that only 0.25–1.5% (28–165) of the total expressed genes (10,013) were uniquely expressed in a single developmental stage, whereas 83.6% (9,198) was shared in all stages.
저자
Hong-Il Kim [ Department of Applied Biochemistry, Konkuk University, Chung-Ju, Korea ]
Chang-Soo Lee [ Department of Applied Biochemistry, Konkuk University, Chung-Ju, Korea ]
O-Chul Kwon [ Department of Applied Biochemistry, Konkuk University, Chung-Ju, Korea ]
Won-Sik Kong [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Korea ]
Young-Jin Park [ Department of Applied Biochemistry, Konkuk University, Chung-Ju, Korea ]
