Earticle

초록

영어

The purpose of the study was to investigate an effect of water temperature on a non- specific immune response and mortality of tilapia, Oreochromis niloticus, following a bacterial infection. Seventy five tilapia acclimated to 25℃ were then transferred at 16 and 36℃, and examined for non-specific immune responses over 12-96 h. Respiratory burst activity was reduced significantly in the group of fish cultured at 16 and 36℃ over 24-96 h, whereas phagocytic activity decreased significantly in the group of fish reared at a low temperature (16℃) over 12 and 24 h and high temperatures (36℃) over 12-96 h. Lysozyme activity diminished significantly in the group of fish transferred to 16℃ over 12-48 h, but increased significantly in the group of fish at 36℃ over 48 and 96 h. Alternative complement pathway (ACH50) decreased significantly when transferred to 16℃ after 12 h, but increased significantly when transferred to 36℃ after 24 h. In a challenging test, 30 tilapia reared at 25℃ were injected intraperitoneally with Streptococcus iniae at a dose of 2x107 cfu/fish, and then reared onward at water temperatures of 15, 25 (control), and 36℃. Over 12-96 h, the cumulative mortality of S. iniae-injected fish held in 16 and 36℃ was significantly higher than that of injected-fish held in 25℃ In conclusion, transfer of tilapia from 25℃ to low temperature (16℃) after 12 h, and transfer of fish from 25℃ to high temperature (35℃) reduced their immune capability. Furthermore, tilapia under temperature stress at 16 and 36℃ from 25℃ decreased its resistance against S. iniae

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 실험어
  2. 포르말린 처리된 Escherichia coli
  3. 호흡폭발 분석
  4. S. iniae의 배양
  5. S. iniae에 대한 틸라피아의 감수성에 미치는 수온의 영향
  6. 면역지표에 미치는 수온의 영향
  7. 백혈구의 분리
  8. 비 특이적 면역세포 반응 측정
  9. 비 특이적 체액성 반응측정
  10. 통계처리
 Ⅲ. 결과 및 고찰
 Ⅳ. 요약
 사사
 Ⅴ. REFERENCES

저자

• 최상훈 [ Sanghoon Choi | 군산대학교 수산생명의학과 ] Corresponding author

참고문헌