Earticle

초록

영어

Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specific DNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilic strain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basis of the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearly distinguish the psychrophilic strains from the control strains.

목차

ABSTRACT
 공시균주 및 저온적응성 계통 선발
 마커 탐색을 위한 RAPD
 저온성 계통에 특이적인 PCR 산물의 Cloning 및 염기서열 분석
 SCAR marker 디자인
 적요
 감사의 글
 참고문헌

저자

• 김수철 [ Su Chul Kim | 경남과학기술대학교 제약공학과 ]
• 황혜성 [ Hye Sung Hwang | 경남과학기술대학교 제약공학과 ]
• 조윤진 [ Yun Jun Cho | 경남과학기술대학교 제약공학과 ]
• 김혜수 [ Hye Su Kim | 경남과학기술대학교 제약공학과 ]
• 류재산 [ Jae-San Ryu | 경상남도농업기술원 친환경연구과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ] Corresponding author

참고문헌