This study was carried out to optimize the conditions for a chemotaxonomic classification of Ganoderma species. The mycelia of Ganoderma species were extracted with 100% MeOH, and the concentrated extracts were further purified and partitioned with column chromatography (HP20) and n-BuOH, respectively. From the result of high-performance liquid chromatography (HPLC), the constituents of the samples were efficiently separated with a Chemco Pak C18 column (250 mmX4.6 mm) by linear gradient elution using water and acetonitrile as mobile phase components at a flow rate of 0.8 ml/min and detector wavelength at 210 nm. However, the samples without purification or partition were not detected the characteristic peaks. This profile could be used to classify and identify the various Ganoderma species.
목차
ABSTRACT 서론 재료 및 방법 결과 적요 및 고찰 감사의 글 참고문헌
저자
윤대은 [ Dae-Eun Yoon | 건국대학교 글로컬캠퍼스 의료생명대학 의생명화학과 ]
박영진 [ Young-Jin Park | 건국대학교 글로컬캠퍼스 의료생명대학 의생명화학과 ]
권오철 [ O-Chul Kwon | 건국대학교 글로컬캠퍼스 의료생명대학 의생명화학과 ]
남재영 [ Jae-Young Nam | 건국대학교 글로컬캠퍼스 의료생명대학 의생명화학과 ]