Earticle

초록

영어

Pholiota species were collected from different geographical regions of the world. Genetic diversity and phylogenetic relationships were analyzed by rDNA-ITS sequences and RAPD polymorphism. The sizes of rDNA-ITS PCR amplicons of Pholiota spp. varied from 233~271, 158~223 and 174~219 bp, respectively. A phylogenetic tree was constructed on the ITS region sequences and Pholiota strains were classified into 8 clusters. Twenty strains in seven Pholiota spp. were classified into seven clusters by RAPD polymorphism using 15 arbitrary primers. Our experimental results suggested that rDN-ITS and RAPD analysis are useful tool for classifying Pholiota spp. and strains.

목차

ABSTRACT
 서론
 재료 및 방법
  실험 버섯 균주
  버섯균사체 배양
  DNA의 추출
  rDNA ITS영역의 PCR 증폭
  DNA 염기서열 결정
  DNA 염기서열 및 유연관계 분석
  RAPD 분석
 결과 및 고찰
  ITS 영역의 염기서열을 이용한 유연관계 분석
  RAPD를 이용한 계통분석
 적요
 감사의 글
 참고문헌

저자

• 권운혁 [ Woon-Hyuk Kwon | 제물포고등학교 ]
• 박혁 [ Hyuk-Park | 제물포고등학교 ]
• 백민재 [ Min-Jae Baek | 제물포고등학교 ]
• 조우진 [ Woo-Jin Cho | 제물포고등학교 ]
• 최우정 [ Woo-Jeong Choi | 제물포고등학교 ]
• 안치범 [ Chi-Beom Ahn | 제물포고등학교 ]
• 신도빈 [ Do-Bin Shin | 인천대학교 생명과학부 ]
• 이태수 [ Tae-Soo Lee | 인천대학교 생명과학부 ] Corresponding author

참고문헌