Earticle

초록

영어

Sparassis crispa is a medicinal mushroom, which has been reported to have anti-cancer effect. In this study, we designed to investigate the effects of Sparassis crispa extracts on the production of nitric oxide (NO) in LPS-stimulated RAW264.7 cells. The pre-treatment of the extracts prior to add LPS in RAW264.7 cells suppressed NO production and iNOS expression at protein and mRNA levels. The phosphorylation of IĸBα was inhibited by the extracts, which was induced through suppressing the activation of NF-ĸB. Sparassis crispa extracts showed the effect on the down-regulation of STAT-1 activation in a dose-dependent manner. In LPS-stimulated RAW264.7 cells, NF-ĸB was translocated into the nucleus, while the treatment of Sparassis crispa extracts induced to sequestered NF-ĸB in the cytosol. These experimental results determined that Sparassis crispa extracts play a inhibitory role in inflammatory reactions via regulating NO production, which suggests potential as a component of inflammatory drugs.

목차

ABSTRACT
 서론
 재료 및 방법
  실험재료 및 추출물 조제
  세포 배양
  꽃송이버섯 추출물의 세포독성 측정
  꽃송이버섯 추출물의 NO 생성 저해 효과 측정
  Reverse transcriptase-polymerase chain reaction (RTPCR)을 이용한 iNOS의 mRNA 발현 분석
  Western blot을 이용한 iNOS의 단백질 발현 분석
  Immunofluorescent microscopy
  통계학적 분석
 결과 및 고찰
  꽃송이버섯 추출물의 세포생존율에 미치는 영향
  꽃송이버섯 추출물의 NO 저해능
  꽃송이버섯 추출물의 iNOS 단백질과 mRNA 발현 조절 효과
  꽃송이버섯 추출물의 iNOS 전사인자 활성조절 효과
  꽃송이버섯 추출물에 의한 NF-ĸB의 세포내 위치전환 억제 효과
 적요
 참고문헌

저자

• 최우석 [ Woo-Suk Choi | 부경대학교 자연과학대학 미생물학과 ]
• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 유영복 [ Yoo Young Bok | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 노형준 [ Noh Hyung Jun | 농촌진흥청 국립원예특작과학원 인삼특작부 ]
• 김군도 [ Gun-Do Kim | 부경대학교 자연과학대학 미생물학과 ] Corresponding author

참고문헌