Earticle

초록

영어

The role of Fatty acid binding protein 4 (FABP4) gene is critical for lipid metabolism and for maintaining homeostasis in adipocytes. Association between Hanwoo carcass traits and FABP4 gene g.7516G>C SNP has been reported previously, however, its association and how does it influence Korean brindle and black cattle has not been demonstrated and established till date. For this purpose, the study was planned to analyze the SNP association (Single Nucleotide Polymorphism) (g.7516G>C) in FABP4 gene and gather genetic information on economic traits of Korean brindle cattle and black cattle. As per sequence of bovine FABP4 gene available (Genbank accession No. NC_007312.4), one pair of primers (5’-ATA TAG TCC ATA GGG TGG CAA AGA-3’ and 5’-AAC CTC TCT TTG AAT TCT CCA TTC T-3’) was designed to amplify a 452bp product of the FABP4 gene including the region of 7417–7868. The SNP, detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) methods using restriction enzyme MspA1I, was genotyped in 117 animals of brindle cattle and 24 animals of black cattle population. Statistical analysis revealed that the FABP4 genotype significantly (p<0.05) affect with carcass weight, but there was no significant association with any other economic traits was observed in brindle as well as in black cattle. In conclusion, these results suggested that SNP (g.7516G>C), located in FABP4 gene, could be used as important DNA marker of economic traits in Korean brindle cattle. Furthermore, we suggest that additional samples needs to further analysed to make related data exclusively authentic.

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 공시재료, 도체형질자료 및 DNA 추출
  2. PCR-RFLP를 통한 FABP4 유전자 SNP의 유전자형 분석
  3. 통계분석
 Ⅲ. 결과 및 고찰
 Ⅳ. 요약
 Ⅴ. References

저자

• 이승규 [ Seung-Kyoo Lee | 강원대학교 동물생명과학대학 ]
• 이영섭 [ Young-Sub Lee | 강원대학교 동물생명과학대학 ]
• 박새롬 [ Sairom Park | 강원대학교 동물생명과학대학 ]
• 김훈 [ Hun Kim | 강원대학교 동물생명과학대학 ]
• 최소영 [ So-Young Choi | 강원대학교 동물생명과학대학 ]
• 이지연 [ Ji-Yeon Lee | 강원대학교 동물생명과학대학 ]
• 김기범 [ Ki-Beom Kim | 축산물품질평가원 ]
• 박종운 [ Jong-Woon Park | 축산물품질평가원 ]
• 최정우 [ Jung-Woo Choi | 캐나다 궬프대학교 ]
• 이학교 [ Hak-Kyo Lee | 한경대학교 생명공학과 ]
• 이성진 [ Sung-Jin Lee | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌