Earticle

초록

영어

To develop new variety of oyster mushroom, 63 intra-specific hybrids between the strain Suhan and #Nongi201 were developed using hyphal anastomosis technique in 2004. The Po2008-275 hybrid between the dikaryon strain 04-154(Suhan x #Nongi201) and the monokaryon strain derived from ASI2487 were developed using hyphal anastomosis in 2008. The Po2008- 275 was shown the best cultural characteristics, selected to be a new variety and named as ‘Guseol’. The new commercial strain, ‘Guseol’ had dark grey pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of ‘Guseol’ were of an excellent quality in that not only the stipe was thick and long but also the pileus was small and hard. The optimum temperatures for mycelial growth and fruiting body development were 25~30℃ and 10~16℃, respectively. Time period required for the initiation of the first fruiting body was about 3 to 5 days depending on the temperatures. The shape of fruiting body was thin funnel shape. Fruiting body production per box(43x43x12cm) was about 1545±400.9g which was almost 137% quantity compared to that of parental strain 04-154. Relatively low temperature incubation (11℃) resulted in the development of better quality of ‘Guseol’ mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the mycelial growth of this mushroom were much faster in mushroom complete medium. Similar results were observed with other variety ‘#Chunchu2’. Analysis of the genetic characteristics of the new commercial strain ‘Guseol’ showed a major DNA profile as that of the parental 04-154 when primer URP 1, primer URP 2 and primer URP 5 were used, but different to ‘ #Chunchu2’ that was used as a control. This new variety of the dark grey oyster mushroom had smart and high quality image that corresponds well to “health food”. We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.

목차

ABSTRACT
 서론
 재료 및 방법
  균주 및 배양
  단포자 분리
  계통간 교잡체 (hybrid) 선발
  자실체 특성 검정
  RAPD에 의한 DNA 양상분석
 결과 및 고찰
  육성경위
  고유특성
  가변특성
  자실체 수량성
  재배상 유의점
 적요
 참고문헌

저자

• 유영복 [ Young-Bok Yoo | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author
• 김은정 [ Eun-Jung Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab-Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]

참고문헌