Earticle

초록

영어

This study was carried to identify a correct species and asses genetic diversity within the same species of Grifola spp. preserved in Division of applied Microbiology. Contaminated isolates showed different growth rates, morphology and color of hyphae. We have reconstructed the phylogenetic tree of a select group of Grifola spp. using nucleotide sequences of the internal transcribed spacer region(ITS) region. The phylogenetic tree was constructed by using the neighbor-joining method. PELF primers of 20-mer were used to assess genetic diversity of preserved isolates. Sequence analysis showed that four strains were identified completely different nomenclature. According to the analysis of ITS sequences, the genus Grifola clustered into one group, most of which correlated with species-groups identified by RAPD method. Eight isolates included strain GM01 showed high similarity with Grifola frondosa. All isolates were collected in the Japan(GM01, GM02, GM03) was identified as Grifola frondosa and isolates of the China(GM05, GM06, GM08) was identified as Bjerkandera fumosa, Grifola frondosa and Dichomitus squalens, respectively. RAPD analysis of genetic polymorphisms of genus Grifola showed a very different band patterns on the isolat. As the result of RAPD and ITS region sequences analysis for preserved isolates, it seems likely that 4 isolates of Grifola spp. may be need to reclassify or eliminate from preserved catalogue.

목차

ABSTRACT
 서론
 재료 및 방법
  시험균주 및 배양조건
  염색체 DNA 추출
  rDNA의 ITS 영역 증폭과 유연관계 분석
  RAPD에 의한 다형성 분석
 결과 및 고찰
 적요
 참고문헌

저자

• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author
• 전창성 [ Chang-Sung Jhune | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 정종천 [ Jong-Chun Cheong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 서장선 [ Jang-Sun Suh | 농촌진흥청 국립원예특작과학원 버섯과 ]

참고문헌