Earticle

초록

영어

Sixty strains of Pleurotus ostreatus, white-rot fungi, were screened for production ability of their lignocellulolytic enzymes to selectively wood degradation. That results were shown that all of screened strains were produced lignocellulolytic enzymes on 2nd screening liquid culture medium. However, cellulase activity of selected six strains of P. ostreatus was low in avicel-yeast-peptone liquid culture medium. In the case of xylan degrading enzyme, No. 6 and No. 38 strains produced a xylanase(above 1.0U/ml) and a 1,4-β-xylosidase (above 0.15 U/ml). Examination of the ligninolytic enzyme profiles of selected thirteen strains of the P. ostreatus, in the presence of Remazol Brilliant Blue R(RBBR), were observed that laccase(Lac) activity were earlier reached maximum level(0.8-2.0 U/ ml) and then Mn-dependent peroxidase (MnP) were reached maximum level(0.5-1.5 U/ml) in glucose- yeast-peptone(GYP) medium. On the other hand, activity of lignin peroxidase(LiP) was not detected in this medium. I selected the No. 42 strain of P. ostreatus produced high levels of Mn-dependent peroxidase and laccase based on the screening method.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주
  리그닌-셀룰로오스 분해 효소 측정 배지 조건
  1차 균주선발을 위한 리그닌-셀룰로오스 분해 효소 활성측정 및 배양방법
  2차 균주선발을 위한 리그닌-셀룰로오스 분해 효소 활성측정 및 배양방법
 결과 및 고찰
  느타리버섯 60균주로부터 리그닌-셀룰로오스 분해효소활성 능력의 1차 선발
  느타리버섯 60균주로부터 리그닌-셀룰로오스 분해효소활성 능력의 2차 선발
 적요
 감사의 글
 참고문헌

저자

• 하효철 [ Ha Hyo-Cheol | 대구한의대학교 한방식품약리학과 ] Corresponding author

참고문헌