Earticle

초록

영어

Spent mushroom substrates (SMS) is a by-product remaining after a crop of mushrooms. About four surfactin-producing strains were isolated from SMS (Pleurotus eryngii). Among of them, one isolate, which designated to YJ07, potentially showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium. The biochemical characteristics of the strain YJ07 was similar with Bacillus subtilis and Bacillus amyloliquefaciens by Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis of the strain YJ07 also showed that the strain YJ07 was most closely related to Bacillus amyloliquefaciens with sequence similarity of 99.5%. On the basis of their biochemical characteristics and phylogenetic distinctiveness, the strain YJ07 was classified within the genus Bacillus as Bacillus amyloliquefaciens YJ07. The antifungal compound from B. amyloliquefaciens YJ07 was similar to lipopeptide surfactin from Bacillus subtilis by TLC and HPLC analysis.

목차

ABSTRACT
 서론
 재료 및 방법
  Surfactin 생성 균주의 분리
  Surfactin 생성 균주의 항균활성
  Surfactin 생성 균주의 동정
  분리균이 생성하는 항균물질의 분리
 결과 및 고찰
  새송이버섯 수확 후 배지로부터 surfactin 생성 균주의 분리
  분리균 YJ07의 항균활성
  분리균 YJ07의 동정
  분리균 YJ07이 생성하는 항균물질
 적요
 감사의 글
 참고문헌

저자

• 신평균 [ Pyung Gyun Shin | 농촌진흥청 인삼특작부 버섯과 ]
• 유영복 [ Young Bok Yoo | 농촌진흥청 인삼특작부 버섯과 ]
• 조용운 [ Yong Un Cho | 경남과학기술대학교 제약공학과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ]

참고문헌