Earticle

초록

영어

Trichoderma disease of oyster mushroom has not been effectively detected in the field for testing its resistance against the disease with its varieties. In this study, we investigated the methods to detect its resistance in the laboratory by using media, which enables us to understand the relevant characteristics (e.g., lysis, toxin enzyme, mycelial growth rate). In coculturing with strains of Trichoderma and oyster mushroom, it is possible to observe the difference in the resistance of oyster mushroom against Trichoderma with the phenomena of barrage reaction, overgrowth and lysis. We also observed the inhibition of mycelial growth of oyster mushroom using the dilution method with 48-well plate, but could not observed the inhibition of mycelial growth using the filter paper method of cultural supernatant. In simultaneously culturing both Trichoderma and oyster mushroom, it was possible to detect the inhibition of the mycelial growth of oyster mushroom, but Trichoderma mycelium did not overgrow against oyster mushroom. We found that the pathogenicity was efficient in using solid medium with the phenomena of overgrowth and lysis by inoculating Trichoderma on top of mycelia of oyster mushroom. In conclusion, the methods (e.g., coculture method, dilution method with 48 -well plate, post-inoculation method) are recommended to detect the resistance of oyster mushroom against Trichoderma disease.

목차

ABSTRACT
 서론
 재료 및 방법
  시험방법 및 공시균주
  대치배양
  배양여액
  분활 petri dish
  배양후 접종
 결과 및 고찰
  대치배양
  배양여액
  분활 petri dish
  배양후 접종
 적요
 참고문헌

저자

• 전창성 [ Chang-Sung Jhune | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ] Corresponding author
• 윤형식 [ Hyung-Sik Yun | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 정종천 [ Jong-Chun Cheong | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 장갑열 [ Kab-Yel Jang | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]

참고문헌