Earticle

초록

영어

This study was carried to identify a correct species and asses genetic diversity within the same species of Trametes spp. preserved in Division of applied Microbiology The morphological and cultural characteristics of preserved strains were observed through microscope and investigated on PDA, respectively. Contaminated isolates showed different growth rates, morphology and color of hyphae. We have reconstructed the phylogenetic tree of a select group of Trametes spp. using nucleotide sequences of the internal transcribed spacer region(ITS) region. The phylogenetic tree was constructed by using the neighbor- joining method. PELF primers of 20-mer were used to assess genetic diversity of preserved isolates. Sequence analysis showed that five strains were different species and six strains were identified completely different nomenclature. According to the analysis of ITS sequences, the genus Trametes clustered into four distinct group, most of which correlated with species-groups identified by RAPD method. Seven isolates included TM 01 strain showed high similarity with Trametes versicolr, TM 07 and TM 10 high similarity with Trametes gibbosa, and TM 05 high similarity with Trametes elegans. But isolates collected in the United States was identified as T. junipericola. T. gibbosa and T. versicolor by RAPD analysis of genetic polymorphisms showed a very different band patterns and these strains showed different band patterns on areas. As the result of RAPD and ITS region sequences analysis for preserved isolates, it seems likely that 11 isolates of Trametes spp. may be need to reclassify or eliminate from preserved catalogue.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주 및 배양조건
  염색체 DNA 추출
  rDNA의 ITS 영역 증폭과 유연관계 분석
  RAPD에 의한 다형성 분석
 결과 및 고찰
 적요
 참고문헌

저자

• 이찬중 [ Chan-Jung Lee | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding author
• 전창성 [ Chang-Sung Jhune | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 정종천 [ Jong-Chun Cheong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오진아 [ Jin-A Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 한혜수 [ Hye-Su Han | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 엄나나 [ Na-Na Um | 농촌진흥청 국립원예특작과학원 버섯과 ]

참고문헌