Genomic DNA prepared from fruitbdies of 25 Grifola frondosa strains were amplified with the ITS primers and the PCR reaction products were enzymed. The ITS bands have same electrophoresis patterns but part of them have different restriction enzyme cutting site. These strains were divided into three broad categories. SRAP amplification employing 47 SRAP primer pairs were carried on and 138 polymorphic bands were detected. The phylogram tree showed that: ten strains were differentiated from the others effectively and fifteen strains have no obvious differences between each other. The results implied that the ITS-RFLP and SRAP markers were effective methods for strains identifica tion and germplasm evaluation but other markers were also needed to be used in conjunction.
저자
Zhang Meiyan [ Key Laboratory of Applied Mycological Resources and Utilization Ministry of Agriculture, Shanghai Research Center for Edible Fungi Biotechnology and Engineering, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Fisheries University, Shanghai 200090, P.R. China ]
Shang Xiaodong [ Key Laboratory of Applied Mycological Resources and Utilization Ministry of Agriculture, Shanghai Research Center for Edible Fungi Biotechnology and Engineering, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Fisheries University, Shanghai 200090, P.R. China ]
Tan Qi [ Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Research Center for Edible Fungi Biotechnology and Engineering, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Fisheries University, Shanghai 200090, P.R. China ]
Li Yu [ Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Research Center for Edible Fungi Biotechnology and Engineering, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Fisheries University, Shanghai 200090, P.R. China ]
