Earticle

초록

영어

Agaricus blazei Murill is a important medicinal mushroom for a powerful immune system builder and tonic. Currently, it is known about a new disease phenomenon that appears to be occurring on a number of mushroom farms. We described a straightforward approach in which molecular methods was used to survey the presence of potentially endo- and epiphytic bacteria infected with the Agaricus blazei. The 16S rDNA was amplified with universal eubacterial primers directly from pure cultures of Agaricus blazei mycelium and fruit body. The 16S rDNA sequences were almost identical (96 to 97% similarity), and phylogenetic analysis placed them in a single unique rRNA branch belong to the uncultural bacterium phylogroup. PCR detection of uncultural bacterium in the malformed tissues of Agaricus blazei were carried out by using 16S rRNA sequenced specific probe. It was strongly amplified at the malformed pileus region of fruit body and also spore print was impossible.

목차

ABSTRACT
 서론
 재료 및 방법
  공시균주 및 PCR 프라이머 제작
  Genomic DNA 분리
  PCR
  Partial cloning 및 sequencing
  조직부위별 PCR
 결과
  16S rDNA 영역을 이용한 기형버섯의 PCR 검정
  기형버섯의 부위별 PCR 검정
  양송이버섯에서 배양불능세균의 동정
 고찰
 적요
 참고문헌

저자

• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 박윤정 [ Yun-Jung Park | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 유영복 [ Young-Bok Yoo | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab-Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오세종 [ Se-Jong Oh | 국립농업과학원 유전자원센터 ]
• 이금희 [ Keum-Hee Lee | 농수산식품부 국립식물검역원 ] Corresponding author

참고문헌