Human GH (hGH) has been available over 40 years for the treatment of children with GH deficiency. Human growth hormone (hGH) is mainly produced in the somatotrophic cells of the pituitary in brain and is the product of the GH-N gene. Among the edible mushrooms, the king oyster mushroom (Pleurotus eryngii ) is one of the most popular mushrooms in Asia, Europe and North America. The increasing popularity of P. eryngii among consumers is due to its flavor, texture and shelf life. We report a modified Agrobacteriummediated method for the efficient transformation of hGH2 in Pleurotus eryngii . The binary vector pCAMBIA1304 was used for the initial transformation and detected by GUS and GFP. Infiltrated samples transformed with pCAMBIA1304 showed a wider GUS response than the co-cultivated in the 50㎍/㎖ hygromycin selection medium. This transformation technique offers new prospects for the production of useful protein in the genetically improved mushroom.
저자
Bong-Suk Choi [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Corresponding author
Seung Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Se-Eun Park [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Hoe-Chang Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Jun-Hui Choi [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Sang-Jun Chung [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
Bong-Su Kim [ Callus Co., 1 O Ryong-dong, Gwangju 500-712, Republic of Korea ]
Nam Jun Chung [ Callus Co., 1 O Ryong-dong, Gwangju 500-712, Republic of Korea ]
Sung-Jun Kim [ Department of Biotechnology, BK 21Research Team for Protein Activity Chosun University ]
