Earticle

초록

영어

The stromatal forms of T. fuciformis and the mycelia of Hypoxylon sp. were collected. The DNA sequence in the ITS region of the 5.8S ribosomal genes of isolated strain KG103 was very similar to that of T. fuciformis AF042409 with a homology of over 98% in the EMBL/GenBank database through BLAST searching. A second isolate, No KG201, one of the symbiotic strains for cultivating T. fuciformis also exhibited high homology with Annulohhypoxylon stygium AJ390406. Potato Dextrose Medium exhibited the best mycelial growth of 14 ㎜/14 days and 85 ㎜/14 days for T. fuciformis and its symbiotic fungi, respectively. Optimum culture conditions for the micelial growth were pH 5 at 25℃. For the optimization of artificial cultivation of T. fuciformis in bottle with sawdust medium, several conditions such as type of sawdust, supplements, pH, moisture content, and incubation temperature were investigated. T. fuciformis and symbiotic fungi showed fast mycelial growth on corn cob media (77 and 52%) followed by oak tree sawdust and cotton seed meal. The optimal temperature for mycelial growth of T. fuciformis and symbiotic fungi on corn cob media was 25 ℃ at 55% of moisture content.

목차

ABSTRACT
 서론
 재료 및 방법
  희목이버섯균과 공생균의 분리 및 동정
  흰목이버섯과 공생균의 배양
  흰목이버섯균과 공생균의 혼합종균 제조방법
  대량 연중생산을 위한 재배용기 개발 및 병속 재배법 개발
 결과 및 고찰
  희목이버섯과 공생균의 특성
  ITS영역 5.8S rDNA sequencing을 이용한 흰목이버섯균과 공생균의 동정
  T. fuciformis KG 103과 A. stygium KG 201의 균사체 배양최적화
  희목이버섯균과 공생균의 배양 최적 pH
  흰목이버섯 대량생산을 위한 배지최적화 및 용기개발
  용기내 재배를 위한 T. fuciformis KG 103, A stygium KG 201의 혼합종균제조
  흰목이버섯 용기내 재배 주재료 선발
  흰목이버섯 용기내 재배법 배지의 최적 수분함량
 적요
 감사의 글
 참고문헌

저자

• 최성우 [ Sung Woo Choi | 수원대학교 ]
• 장현유 [ Hyun-You Chang | 버섯연구회 ]
• 윤정원 [ Jeong Weon Yoon | 수원대학교 ]
• 이찬 [ Chan Lee | 중앙대 ] Corresponding author

참고문헌