Earticle

초록

영어

The enormous production of spores by the fruitbodies in the cultivation of oyster mushrooms (Pleurotus ostreatus) is develop an allergy with symptoms similar to an “extrinsic allergic alveolitis (EEA)”. the sporeless strain would noy only benefit health of mushroom workers but also reduce the risk of viral infections on the mushroom farms. For the development of a sporeless strain of P. ostreatus we used strain ASI 2069. This non-commercial strain is completely nonsporulating. We have recovered both nuclear types of strain ASI 2069 as monokaryons (hereafter referred to as neohaplonts) by protoplasting the mycelium. Crosses between neohaplonts and SSI's(single spore isolates) obtained from a sporulating commercial strain ASI 2180 yielded fruitbodies that isolated 128 strains. 13 excellent strains are selected from 30 bred strains by quality of fruitbodies and spore number. Among 13 excellent strains, G192 strain is chose finally to high yield and sporelessness.

목차

ABSTRACT
 서언
 재료 및 방법
  균주 및 배양배지
  무포자형성 자연돌연변이 균주의 선발
  원형질체 재생에 의한 단핵화 균주 분리
  균주간 교잡
  교잡체의 자실체 및 포자형성능 검정
 결과 및 고찰
  무포자형성 균주의 선발 및 특성 검정
  소포자형성 교잡균주 육성
 적요
 인용문헌

저자

• 신평균 [ Pyung-Gyun Shin | 농촌진흥청 농업과학기술원 식물영양과 ] Corresponding author
• 오세종 [ Se-Jong Oh | 응용미생물과 ]
• 유영복 [ Young-Bok Yoo | 응용미생물과 ]

참고문헌